Introduction: It is known that the activity of intestinal enzymes involved in the digestion of carbohydrates increases in diabetes mellitus. An increase of intestinal brush bolder maltase, sucrase, and lactase activity was shown in experimental diabetes and hyperglycemia. To reduce the activity of intestinal carbohydrates various herbal preparations are used. Objective: This work aims to study the effect of prenatal on the activity of intestinal disaccharidases in alloxan-induced diabetic rats. Material and Methods: White outbred rats were divided into the control and experimental groups to receive a single injection of saline, or alloxan monohydrate (150 mg/kg) (intraperitoneal) respectively. Further, experimental animals with high glucose levels were divided into the experiment I and experiment II groups. Control and experiment 1 group rats were injected with saline. Experiment 2 group rats were injected with prenatal (5mg/kg/24 h) for 30 days. The glucose level and intestinal disaccharidases activity were determined by using a glucometer and special kits (Human, Germany) respectively. Results: It was found that in experimental diabetes, the activity of the small intestine carbohydrates sharply increased. Injection of prenalon to alloxan-induced diabetic rats resulted in stabilizes of glucose level and normalizing of the activities of intestinal sucrase, maltase, and lactase. Conclusion: Administration of prenatal to the alloxan-induced diabetic rats led to the restoration of blood glucose levels and the activity of intestinal disaccharidases.