Background: Assam along with the other North Eastern States i.e., Manipur, Mizoram, Sikkim, Tripura, Arunachal Pradesh, Meghalaya and Nagaland contribute 50% of India__ampersandsignrsquo;s entire plant biodiversity. Phytochemicals are bioactive chemical compounds obtained from the plants and are widely used as traditional herbal medicine. These herbal medicines are used by the local peo ple to cure various diseases. Objectives: The objective of the present study is the phytochemical as well as antimicrobial activity of ten medicinal plants i.e., VitexnegundoL., OldenlandiacorymbosaL., Centellaasiatica(L.) Urb., Costusspeciosus(J. Koenig) Sm., OcimumsanctumL., Piper nigrumL., MimusapudicaL., PhlogacanthusthyrsiflorusNees., Andrographispaniculata(Burm. F) NeesandIpomoea aquatic Forssk. Methodology: Taxonomy of the studied plants were confirmed using the existing tools. Plant extracts were subjected to phytochemical and antimicrobial testing based on the standard protocol previously described. Results: It was found that steroids were present in the leaves of Costusspeciosus, Piper nigrum, Mimusapudica, Ipomoeaaquatica. Terpenoids were most commonly observed in the leaves of Oldenlandiacorymbosa, Piper nigrum, Phlogacanthusthyrsiflo rus, Andrographispaniculata. The flavonoids were absent in the leaves of Ocimum sanctum. There were mixed results for the presence of the phytochemicals such as tannins, glycosides, coumarins etc. The antimicrobial study was carried out by the disc diffusion method against the human pathogenic bacteria. It was found that the selected medicinal plants are good sources of antimicrobial against pathogens like Salmonella typhi, E. coli and Klebsiella pneumonia. Conclusion: It was concluded that the plants studied were rich in phytochemicals with significant pharmacological and medicinal properties.