Introduction: An anterior reference point is a physical requirement for orienting casts in three-dimensional space in an articula tor. Different anterior points of reference for different articulators have been proposed. Aims: To evaluate four different anterior reference points and their effect on the protrusive condylar guidance angle obtained in a semi-adjustable articulator. Methodology: A total of 20 subjects were selected with Class I dental relationship. Face bow records were taken for orbitale and orbitale minus 7mm as the anterior points of reference. Another face bow record was taken without considering any point on the face and orienting the face bow on the articulator with the superior annular notch and inferior annular notch on the incisal guide pin as an anterior point of reference. Protrusive bite registration was made with the help of an anterior jig. Programming of the articulator was done with the protrusive bite record to evaluate condylar guidance angle. Two lateral cephalograms were made in maximum intercuspation and protrusive position with the help of an anterior jig. Cephalograms were traced and superimposed to obtain condylar guidance angle. Descriptive statistics were performed to obtain the means and standard deviation of all the sample sizes. Paired __ampersandsignlsquo;t__ampersandsignrsquo; test was performed between radiographic readings and condylar guidance angle obtained. Result: This study showed that the protrusive condylar guidance values registered for inferior annular notch and Orbitale as anterior points of reference were statistically significantly closer to the radiographic values. Conclusion: The orbital __ampersandsignamp; inferior annular notch mounts the maxillary cast more accurately to the Frankfort Horizontal Plane when they are used as the anterior points of reference.