Introduction: Urinary tract infection (UTI) is a spectrum of disease caused by microbial invasion of the genitourinary tract that extends from the renal cortex of the kidney to the urethral meatus. The predisposing factors for UTI can be anatomical abnormalities of the urinary tract, Impaired local protective mechanisms, deteriorating immune status, cognitive impairment, comorbid conditions such as diabetes, malignancy, steroid use and chronic debility. Urine serves as an excellent culture medium, to avoid false-positive results transportation should be immediate. Sample should be stored in a refrigerator at 4oC for 48hrs if there is a delay of more than one to two hours. Aim: Here is to study the drug sensitivity pattern in diabetic and non-diabetic patients with urinary tract infections. Method: That we have adopted for our research is carried on patients diagnosed with Urinary tract infection with or without a history of type 2 Diabetes mellitus who were admitted in medical wards in Krishna Hospital and Medical Research Centre, Karad during the study period of 18 months. This study was conducted over 18 months. (October 2018 to March 2020). Ethical clearance from the college and university committee was taken. Result: In the present study, a total of 90 patients diagnosed with urinary tract infections were observed. Among them 45 patients were diabetics and 45 patients were non-diabetics. In the current study, the diabetic group had 26 females (57.78%) and 19 were males (42.22%) and the non-diabetic group had 29 females (64.44%) and 16 males (35.56%) males. Conclusion: Urinary tract infection is one of the common infections requiring hospitalization. In the present study female gender was predominantly affected with urinary tract infection in both groups (i.e. Diabetics and non-diabetics).