Introduction: The study was performed to check the efficacy of intraocular pressure (IOP) measured by the Perkins applanation tonometry (PAT) over the gold-mann applanation tonometry (GAT) and to present and investigate the results, applying appropriate statistical methods. Aims: To comparative intraocular pressure measurement between gold-mann applanation tonometer and Perkins applanation tonometer in glaucoma patients. Methodology: Total 100 eyes (right eyes) of 100 patients underwent both types of instruments for measuring the intraocular pressure IOP which are gold-mann applanation tonometry (GAT) and Perkins applanation tonometry (PAT). Data collected were used for statistical analysis. Student t-test was used to find significance level between two study groups and p __ampersandsignlt; 0.05 was considered as the significance level. Result: Total 100 eyes of 100 patients were included. Intraocular pressure (IOP) values taken from the Gold-mann applanation tonometry (GAT) (which is a definitive measure) were ranged between 10-30 mmHg. Based on IOP all the population groups were divided into three groups the first group with IOP __ampersandsignlt; 18 mmHg including 38 patients, the second group of 32 patients with IOP ranged between 18-24mmhg and the third group of patients with IOP __ampersandsigngt;24mmhg. The mean intraocular pressure (IOP) measured by gold-mann applanation tonometry (GAT) in our study was 22.45 mmHg. The mean intraocular pressure (IOP) measurements done by Perkins applanation tonometry (PAT) was 22.12 mmHg. The mean difference in the measurement of intraocular pressure (IOP) between the two instruments in our study was found 0.33 mmHg. By studying both the methods of measuring IOP it has been found in our study that there is not much significant difference in measuring IOP with both the methods it__ampersandsignrsquo;s only 0.33mmhg. Conclusion: The intraocular pressure IOP measured from the Perkins applanation tonometer is highly comparable with the intraocular pressure IOP measured from the Goldman applanation tonometry (GAT).