Introduction: Biofilm production is an important phenomenon by bacteria such as E.coli that contribute to multiple drug resistance. The green synthesis of nanoparticles (AgNPs) with the use of plants and vegetables has more advantages because they are safe to handle and easy available. Aim: In the present study silver nanoparticles were synthesized by an eco-friendly and cost-effective method using Raphanus sativus [leaf]. This acts as reducing and stabilizing agents. Methods: The formation of Ag NPs has been characterized and confirmed through Scanning Electron Microscope [SEM], X Ray Diffraction [XRD], Fourier Transform Infra-Red [FTIR] and Ultraviolet [UV] visible absorption spectrum. The key step in this process is the identification of virulence determinants that may serve as targets for vaccine and drug development. Results: The phytochemical investigation uncovered the presence of rich measures of Poly phenols and other biochemicals in these concentrates, which fill in as decreasing and capping agents for converting silver nitrate into AgNPs. FTIR confirmed the role of biomolecules in the bioreduction and efficient stabilization of AgNPs. UV__ampersandsignndash;Visspectra showed a band of around 279 nm, which implies the characteristics of AgNPs. XRD patterns have shown the crystalline nature of the synthesized AgNPs with fcc structure. SEM analysis revealed the spherical shape of the synthesized AgNPs with an average particle size of 8 nm. Effectiveness of the green synthesized nanoparticles by Aqueous extract against UTI e.coli strain was measured in terms of zone of inhibition in millimetres. Conclusion: These green synthesized silver nanoparticles had significant antibacterial activity. The present study is supportive evidence that Leaf of R. sativus, generally wasted, have important medicinal constituents.