Introduction: Cardiovascular autonomic neuropathy (CAN) is a chronic complication of diabetes with life-threatening complications ranging from resting tachycardia to sudden death. Considering hyperglycaemia as the main etiopathogenic factor of CAN, its detection in the prediabetic stage will have a huge impact in delaying the development of severe forms of CAN and its complications. Objective: This study had been planned to evaluate cardiac autonomic functions in prediabetics by cardiac autonomic reflex tests. Method: This cross-sectional study was carried out in individuals with newly detected prediabetics and normoglycemic controls attending the medicine outpatient department for routine checkups. Anthropometric indices, blood pressure and serum lipids were assessed. Cardiac autonomic reflex tests were performed and individuals were divided according to Ewing__ampersandsignrsquo;s criteria of CAN. The cardiovascular risk factors were compared to see their association with CAN. Result: In this study CAN was found in 15.2% prediabetics and 3.6% normoglycaemic age and gender-matched controls (p=__ampersandsignlt;0.0001, S). No significant association of CAN with cardiovascular risk factors like Body Mass Index, waist-hip ratio, hypertension and serum lipids was found. Conclusion: As per multivariate regression analysis results, prediabetic status was considered to be the sole risk factor for the presence of CAN.