Objective: Many studies have shown the presence of eosinophilia in hookworm infection. But so far detailed study was not done to know about the absence of eosinophilia in hookworm infection. Hence a detailed study was done to know about the absence of eosinophilia in hookworm infection diagnosed by doing upper gastro-intestinal endoscopy in our institute. Methods: A study of 1137 patients who had undergone upper gastro-intestinal endoscopy for a period of four years and eight months from May 2009 to December 2013 was carried out in our institute to know about the absence of eosinophilia in hookworm infection diagnosed by doing upper gastro-intestinal endoscopy in our institute. Results: Out of these 1137 patients, 14 patients found to have hookworms in duodenum while doing upper gastro-intestinal endoscopy were taken into consideration for our study. Out of these14 patients with hookworms in duodenum, as many as 10 patients with hookworms in duodenum were found to have eosinophilia [71%]. But 4 patients with hookworms in duodenum di__ampersandsignnbsp; nt have any eosinophilia[29%]. Out of these 4 patients without eosinophilia with hookworm infection, 3 patients without eosinophilia with hookworm infection had anaemia[75%]. Conclusion: Hence, eosinophilia can sometimes be absent in hookworm infection. But 3 out of 4 patients without eosinophilia with hookworm infection had anaemia in our study. Hence anaemia is an important indicator of hookworm infection which is not associated with eosinophilia.