Introduction: Nitrate is the major concern of groundwater. High concentration arises due to the massive application of chemical fertilizers. It causes various issues Blue Baby Syndrome, respiratory problems etc. Conventional methods are available to remove nitrate, but they can sequester the nitrate and not treat it. Bioremediation is a low-cost technique and is efficient in nitrate removal. Objective: Isolation and identification of nitrate-reducing microbes from the polluted site of Yamuna river. Methods: This paper focuses on microbe__ampersandsignrsquo;s isolationusingBioremediation technique and their identification through Molecular and Biochemical tests. Result: 4 microbes were found to have the potential for remediating nitrate. Microbes were inoculated in nitrate broth and incubated (37?C) under both aerobic and anaerobic conditions. Phenotypical and molecular characterization was done. The consensus sequence was submitted in GenBank and accession numbers were obtained.These were Enterobacteraerogenes (MN252552), Lelliotiaamnigena (MN647560), E. coli K12 (MN754025) and Klebsiella oxytoca (MT457847) respectively. After incubation, these strains showed 48.4%, 34.1%, 42.90% and 33.66% of nitrate remediation under anaerobic conditions while almost negligible under aerobic. Further, these microbes were inoculated in broth containing 1500mg/l nitrate equivalent to the maximum reported nitrate in Yamuna River. E. coli and Enterobacter aerogenes remediated 44% and 46% of 1500mg/l nitrate respectively. Conclusion: These two microbes were found to have moderate potential to remediate nitrate (1500mg/l) which brings novelty to this study. A further detailed study on using microbial consortium to enhance the capability of nitrate remediation will be useful.