Introduction and Objective: Copper oxide (CuO) nanoparticles are one of the most significant transition metal oxides in the burgeoning area of nanotechnology due to their intriguing features. Because of its simplicity, eco-friendliness, and potential as next-generation antibiotics, its synthesis using green chemistry principles is gaining traction. Cost-effectiveness, lower toxicity, and remarkable broad-spectrum antibacterial activity against a range of bacteria through the generation of reactive oxygen species (ROS) and release of copper ions. Materials and Methods: For CuO Nanoparticles synthesis Copper sulfate was used as starting material and its reduction was carried by Coriander Leaf Extract from Cu2+ to Cu0 The synthesized Cu nanoparticles were characterized by UV-Visible, FTIR and XRD methods. Transmission electron microscopy (TEM) demonstrated particle sizes in the range of 10__ampersandsignndash;15 nm. CuO nanoparticles demonstrated antimicrobial activity against a range of Gram-positive and Gram-negative bacteria, including MRSA. Time kill determination assay was done. Results: According to TEM energy dispersive spectroscopy, the copper to oxygen element ratio is 54.18 per cent to 45.26 per cent. Most resistant human pathogenic strains, including Gram-positive and Gram-negative bacteria, showed significant inhibitory action (p0.0001). With a 31.66 mm zone of inhibition, the maximum effectiveness was recorded against Bacillus cereus. The addition of a sub-MIC concentration [broth dilution technique] of nano CuO reduced all populations to zero by 4 h. Conclusion: Studies of CuO nanoparticles suggest the release of ions may be required for optimum killing