Introduction: Crystals are meant for versatile applications and utility in all fields and mainly in electronic and biofields. Aim: Our aim, the present investigation is focused on Bis Glycine Lithium Bromide Monohydrate (BGLBMH crystals), its synthe sis and the various characterizations. Methodology: BGLBMH Macro crystals are put in order by slow evaporation solution growth method and nanocrystal by mill ing method. The Single-crystal XRD, Powder XRD analysis, filter, anti-diabetic and anti-oxidant (AD and AO) studies were performed here. Result: The single-crystal XRD study reveals the macro-crystalline lattice parameters with a, b, c in __ampersandsignAring; as 7.5397, 17.4174, 8.2727 and __ampersandsignbeta; as 118.14o as the system is monoclinic with a space group of P21/c. The macro and nano scales are analyzed for fluorescence spectral activity. The crystals speciality is THG and shows the SHG NLO value of 1.25 times that of KDP because of the strongest H bonds and the bandgap is 3.08 eV which is 403 nm as emission FL value for macro scaling and 397 nm for nano scaling with a bandgap of 3.12 eV. The nano outline of BGLBMH crystals is 250 nm and 34 nm correspondingly for the initial and final one. Conclusion: The BGLBMH have good scope for anti-diabetic by the Glycine, bromide presence and have increased in inhibi tion as concentration increases and the IC value as 37.5 for macro and in a nano form, it is 30.4. Also, the AD - nm variations will have good efficiency when the size of the sample decreases from 250 to 34 nm. The BGLBMH macro and nanocrystals are used in filter applications also as the data are represented and concluded with the inferences and reported with the utilities for electronic and pharma utilities.