Introduction: Rapid and reliable detection of carbapenemase-producing microorganisms is an important element of antimicrobial stewardship. Various phenotypic methods for the detection of these organisms have their limitations. Aims: This study was conducted to assess the performance of the Modified Carbapenem Inactivation Method (mCIM) which is a recent specific phenotypic method described by CLSI 2018 guidelines for the detection of carbapenemase production. Methodology: In the study, mCIM was performed in 80 Gram-negative isolates that were resistant to meropenem by the Kirby Bauer disc diffusion method. Separately, another 20 blaKPC( Klebsiella pneumoniae carbapenemase) polymerase chain reaction (PCR) positive and 15 blaKPC PCR negative Klebsiella pneumoniae isolates were tested by mCIM and their results were compared. Results: The positivity of mCIM was 92.7% and 80% in the case of Enterobacteriaceae and Pseudomonas spp. respectively. The test also showed 100% sensitivity for detecting carbapenemase production in PCR positive Klebsiella pneumoniae isolates harbouring the blaKPC gene. Conclusion: mCIM is a user-specific phenotypic test that is simple and easy to carry out for the detection of carbapenemase-producing microorganisms in microbiology laboratories.