Introduction: Chronic foot infections in patients with diabetes mellitus are a common and difficult problem. The optimal management of these diabetic foot infections requires isolation and identification of the various pathogens and selection of appropriate antibiotic therapy according to the sensitivity patterns. Aims and Objectives: To determine the prevalence of various bacterial pathogens in diabetic foot infections (DFIs). Materials and Methods: A retrospective analysis of the culture of bacterial isolates of pus, pus swabs and tissue samples obtained from diabetic patients admitted in surgical wards at SLIMS, were carried out over 4 years from August 2016 to September 2020. Results: Total growth constituted 131(95%) monomicrobial growth was 107 (78%) and polymicrobial growth was 24(17.5%). In our study, Gram-negative isolates were 77 (79%) with Pseudomonas spp being the most predominant. Most of the Gram-negative bacteria were found to be sensitive to amikacin (56.9%), imipenem (66.6%) and piperacillin-tazobactam (47.1%). Gram-positive isolates were 11 (11.3%) with Staphylococcus aureus being the most predominant. In our study Gram, positive isolates were sensitive to erythromycin (81.8%), ciprofloxacin (81.8%) and cotrimoxazole (54.5%). 100% sensitivity was observed with linezolid, teicoplanin and vancomycin. A total of 21 (27.3%) patients who presented with gangrene were amputated. Conclusion: There is an increase in the prevalence of organisms as Wagner__ampersandsignrsquo;s grade increased with Gram-negative growth being more predominant. Frequent surveillance of antibiotic-resistant patterns would be useful for deciding empiric antibiotic therapy.