Background: India depends upon world market to fulfill their industrial and domestic demand of oil, account huge foreign exchange. Since agriculture in India depend upon monsoon, causes uncertainty. In last few decades, there is rapid increase in demand of oil and their oleo chemicals in industries like plasticizers, lubricants, pharmaceuticals, organic pesticides, agro products etc. Aim: The aim of this study is to explore non-traditional oil sources in order to establish them for various purposes. Methodology: Seeds from four plant species of genus Ipomoea (I. indica, I. nil, I. pestigridis and I. quamoclit) belonging to convolvulaceae family were subjected to various analytical technique to evaluate their physico-chemical properties. Fatty acid methyl esters (FAMEs) were analyzed using chromatographic and spectroscopic techniques to evaluate their fatty acid compositions. Results: The results show that the oil yields from the seeds varied from 7.84% to 14.71%. Linoleic and oleic acids were found major fatty acids in all four seed oils. They show high iodine and saponification values. The all four seed oil show high content of polyunsaturated fatty acids (PUFAs) and P/S index more than 1. Conclusion: Fair oil%, high saponification value and P/S index more than 1, indicated us to use these parameters to establish them as alternate oil sources for various domestic and industrial applications.