Introduction: Abuse is the use of some bad words or treating someone other with harmful treatment that includes mental, physical, sexual, verbal or intellectual maltreatment. Adolescence is the transition age group that includes major changes in life such as social, mental, physical and emotional changes. Thus, the girls are more prone to such conditions and need to be assertive in those particular situations. Assertive training is the procedure of acquiring skills within self and standing for the rights of one__ampersandsignrsquo;s own. The assertiveness training program should be used and taught to adolescent girls as it can help in improving their social skills, self-esteem and concept. Objective: The primary objective of the study was to assess the effectiveness of assertive training programs on knowledge regarding the prevention of abuse among adolescent girls. Methodology: The study setting was MHR DAV Institute of Nursing, Jalandhar, Punjab. A one-group pre-test post-test design was employed to get data collected. The study sample comprised of 100 adolescent girls who are selected through a purposive sampling technique from the selected nursing institution. A self-structured knowledge questionnaire was used to evaluate the knowledge regarding the prevention of abuse among adolescent girls. Assertive Training Program was prepared and administered to the adolescent girls. Results: The findings of the study revealed that after the administration of the Assertive Training Program majority of the adolescent girls 67 (67%) were having average knowledge regarding prevention of abuse among adolescent girls. The mean difference between pre-test and post-test knowledge was 6.06 and the calculated value (11.23) was found to be statistically significant. Conclusion: The study concluded that after the administration of the Assertive Training Program highly significant difference was found between pre-test and post-test knowledge scores regarding prevention of abuse among adolescent girls which was evaluated by a self-structured knowledge questionnaire.