Introduction: Staphylococcus particularly MRSA (Methicilin resistant Staphylococcus aureus) and MR CoNS (Methicilin resistant Coagulase Negative Staphylococcus) are important cause of hospital and community acquired infections. But there are reports of resistance to anti-MRSA drugs particularly vancomycin, teicoplanin, linezolid, tigecyclin which is a cause forconcern. Aim: The present study was conducted to determine the susceptibility of Staphylococci and note the pattern of minimum inhibitory concentration (MIC) of the second line anti staphylococcal drugs in common clinical use. Method: Various culture samples submitted to Department of Microbiology from Sept 2019 to May 2020 and yielding growth of Staphylococcus were included in the present study. Sensitivity pattern was determined by Vitek 2, Biomerioux and MIC of drugs were interpreted as per recent guidelines. Result: 264 Staphylococcus were included in the study of which 39 were Staphylococcus aureus, 72 were S. epidermidis and 67 were S. haemolyticus.Besides these, 46, 14 and 11 strains of S.hominis, S cohinii and S. capitis respectively were isolated. With cefoxitin screen method, 23 (58.9%) isolates were MRSA where asonly 16 (41.03%) were MSSA. Of the S.epidermidis and S.haemolyticus isolated 98.59% and 95.01% were methicillin resistant and all other isolated CONS were methicillin resistant. MRSA isolates had higher degree of resistance in comparison to MSSA to all the antibiotics tested except quinolones and erythromycin.All the S.aureus isolates were 100% sensitive to linezolid, vancomycin, daptomycin, teicoplanin, rifampicin and nitrofurantoin. None of the Staphylococcus showed any creeping of the minimum inhibitory concentration (MIC)of vancomycin. CONS particularly S.epidermidis and S. haemolyticus had a greater degree of resistance to linezolid , daptomycin and teicoplanin than S.aureus.Tigecyclin a reserve drug was the most sensitive antibiotic in all the species. Conclusion: The study highlights high degree of resistance in CONS isolates to all drugs. Linezolid a useful oral drug also showed high resistance to many CONS and few S.aureus strains. Rifampicin and tigecyclin are other useful drugs for Staphylococcus in the present study.