Introduction: Intestinal parasitic infection is a major global health problem, more so in poor and socio-economically deprived communities in the tropics and subtropics. These are among the 10 most common infestations of the world. Intestinal protozoa and helminths flourish in settings characterized by poor sanitation, dirty water, substandard and crowded housing. To determine the prevalence of intestinal parasitic infection in patients, a prospective laboratory analysis of stool samples for intestinal parasites was carried out in a tertiary care hospital. Aim: To determine the prevalence of intestinal parasitic infection in patients, prospective laboratory analysis of stool samples to study the intestinal parasites. Methodology: A total of 2700 symptomatic patients were investigated for intestinal parasites in their stool samples. All stool samples were subjected to direct wet mount, concentration techniques and modified acid-fast staining and looked for the presence of ova and cysts. Results: The prevalence of intestinal parasitosis was 7.2% (195/2700). There was a high prevalence of protozoan parasites (75.4%) than helminthic parasites (24.6%). A total of 8 different parasites were found. E. histolytica (39.3%) was the most common parasite found, followed by G. lamblia (35.7%) and A. duodenale (8.7%). Conclusion: As these parasitic infections are acquired because of poor personal and environmental hygiene, it is necessary to develop effective prevention and control strategies, the most important being health education. The expansion of health services for an improvement in community health must be enforced.