Introduction: The remnant lipoproteins Cholesterol are highly atherogenic, because of their lesser size along with high cholesterol content, and increased residence period in the blood which may not be reflected by the levels of LDL-C. Elevated Remnant lipoprotein cholesterol and decreased level of Nitric oxide is the novels atherosclerotic risk factors that stimulate the immune and inflammatory reactions associated with the progression of coronary heart disease. Aim and Objective: The study aims to assess the role of remnant lipoprotein cholesterol associated with nitric oxide in the development of coronary heart disease. Materials and Methods: This cross-sectional study was conducted in SRM Medical College Hospital and Research Centre on subjects appearing the master health check-up and medicine OP. The study was conducted on 97 CHD patients and 97 healthy control in the age group of 30 to 55 years and was age and sex-matched. After overnight fasting body fluid samples were collected for analysis for Lipid Profile, Nitric oxide was measured by ELISA method and Lipid Profile is measured using Auto Analyser AU480. Statistical analysis was done using Student__ampersandsignlsquo;t__ampersandsignrsquo; test and Pearson correlation analysis for the comparison between two groups. Result: The mean levels of FBG, Total cholesterol, Triglyceride, LDL-C, VLDL, TC/HDL-C Ratio, LDL-C/HDL-C Ratio, RLP-C, were significantly increased in CHD subjects when compared to controls. And the mean Nitric Oxide levels were decreased significantly in the CHD group compared to controls (P__ampersandsignlt;0.001). Conclusion: Decreased levels of Nitric oxide and the elevated levels of RLP-C in the arterial wall impairs endothelial function resulting in the progression of CHD.