Introduction and Objective: Hepatitis C infection (HCV) is one of the important causes of chronic liver diseases (CLD). This causes significant morbidity and mortality in developing countries like India due to its low-level infection control. The destinations of the examination were to investigate parenterally transmitted, HCV in recently analysed haematological disorders, CKD and CLD patients from Madras Medical College and Hospital, Chennai, and further, explore the HCV genotype appropriation in those patients. Materials and Methods: Clinical examination, socio-demographic history, and risk factors exposure and clinical details of each patient were recorded in the proforma. Identification of hostile to HCV antibodies was undertaken using enzyme immunoassay (ELISA KIT). Anti-HCV- seropositive samples were tested for HCV RNA. Hepatitis C virus-ribonucleic acid (RNA) was detected using a Reverse Transcriptase polymerase chain reaction (PCR) assay. HCV genotyping was determined by HCV RT-PCR genotyping test by using the COBAS AMPLICOR LINEAR ARRAY method. Results: Prevention and control of Hepatitis C Virus infection were troublesome as a result of the intricacy present in geological dissemination of HCV and its genotypes, related danger elements and cofactors. HCV type 1 frequently [43/45 = 96% CI (85%- 99%)] more overwhelming in our investigation Conclusion: This study concluded that transfusion of blood and blood products and therapeutic intervention by infected needle are two important preventable modes of spread of hepatitis C virus infection. This made a high risk in children in our country. Improved surveillance and periodic epidemiological studies will have to be undertaken to monitor and prevent this blood-borne virus and its genotype distribution.