A proxy model for optimization of operating conditions (pyrene concentration, biodegradation time and aeration) for biodegradation of pyrene by Corynebacterium sp and Pseudomonas putida was aimed to be investigated. The proxy model for biodegradation of pyrene with activity of Corynebacterium sp and Pseudomonas putida was developed from experimental data using response surface methodology (RSM) with central composite design (CCD) of the design of experiments software. Corynebacterium sp degraded 96.71 % of pyrene at optimal conditions of 68.16 mg/L of pyrene concentration, biodegradation time of 82.57 hours and aeration condition of 3.0125vvm, while Pseudomonas putida degraded 93.84 % of pyrene at optimal conditions of 69.90 mg/L of pyrene concentration, biodegradation time of 84 hours and aeration condition of 3.4995 vvm. The developed proxy model of biodegradation of hazardous pyrene disposal under the stated operating conditions is fit and acceptable for optimization.__ampersandsignnbsp;