Introduction: Cadmium and lead are toxic elements among the heavy metals group. Through water, it enters into the food chain and became hazardous to all living bodies, so its removal is important. Biosorption through Lactic acid bacteria is the most effective technique for the removal of these hazardous metals from water even at very low concentrations. Aims: In the present study, using two strains of Lactic acid bacteria i.e. L. reuteri and L. Plantarum and its exopolysaccharides (EPS) biosorption of Cadmium and Lead were performed due to a well-known toxicant and hazardous to humans and other living bodies. Methodology: Firstly, EPS were isolated from both strains. Biosorption tests were performed using various condition like pH (pH2.0, 4.0, 6.0, 8.0), temperature (37__ampersandsigndeg;C,42__ampersandsigndeg;C and 50 __ampersandsigndeg;C) and contact time (6, 24 and 48 hours). Cadmium and lead sorption were tested in both strains and their Exopolysaccharides through atomic absorption spectrophotometer (AAS). Results: Biosorption of cadmium through EPS was higher as compared to cells in both strains. Lactobacillus reuteri showed 89% biosorption of cadmium through EPS and EPS of Lactobacillus Plantarum showed 51% biosorption of lead. In the case of cells, biosorption of lead was more i.e. 57% through Lactobacillus reuteri and 43% through Lactobacillus Plantarum as compared to cadmium. Conclusion: Exopolysaccharides showed comparatively maximum biosorption as compared to cells. Both strains and their EPS biosorption may be applied in metal detoxification of the environment and different food and pharma industries.