Introduction: Antibiotics were considered the greatest invention in the 20th century. Antibiotic resistance is an alarming issue in developing countries and the major reason contributing to this is the irrational use because of the lack of knowledge. Aim: To understand the level of knowledge and the usage pattern of Antibiotics among Parents of Young Children attending a Tertiary care Hospital Methodology: The present study was a cross-sectional study conducted among the parents of children attending the Pediatric Out-patient department in a tertiary care hospital in Kancheepuram district, Tamil Nadu. A total of 133 participants were surveyed using a semi-structured and a pre-tested questionnaire to find out their understanding of Antibiotics. Results: Among the 133 participants, around 23.4% of parents correctly identified antibiotics are used against bacterial infections, and only 16.6% of people knew about the term antibiotic resistance. About 60% of parents said that they never used leftover antibiotics. People with low socio-economic status were found to have a lack of knowledge regarding antibiotics and they misuse them without knowing the consequences. Conclusion: We need to invest more time in educating and creating awareness regarding the judicial use of antibiotics and to check unprescribed dispensing of antibiotics.