Objective: A detailed study was done to know about the severity of anaemia and its relation with the gender of the patients (male or female patients) with hookworm infection found in the duodenum while doing upper gastro intestinal endoscopy in our institute. Methods: A study of 1259 patients who had undergone upper gastro-intestinal endoscopy for a period of 5 years from May 2009 to April 2014 was carried out in our institute in order to find out the severity of anaemia and its relation with the gender of the patients( male or female patients) with hookworm infection found in the duodenum while doing upper gastro intestinal endoscopy in our institute. Results: 1. Out of these 1259 patients, 14 patients found to have hookworms in duodenum while doing upper gastro-intestinal endoscopy were taken into consideration for our study. 2. Out of these 14 patients with hookworms in duodenum, 9 patients had anaemia. Out of these 9 patients with anaemia, 2 patients were found to have severe anaemia,1 patient had moderate anaemia and 6 patients had mild anaemia. Out of these 14 patients with hookworms in duodenum, 5 patients did not have anaemia. 3. Out of these 14 patients, 6 patients were men and 8 patients were women. Most of the male patients with hookworm infection did not have anaemia. But almost all the female patients with hookworm infection had anaemia. The few male patients with anaemia had only mild or moderate anaemia. Severe anaemia occured only in the female patients and did not occur in any male patient. Conclusion: Hence female patients with hookworm infection were more prone for anaemia than the male patients in our study. .