Background: Bladder outlet obstruction (BOO) is defined as blockage at the neck of the urinary bladder. This is one of the most common conditions in elderly men. BOO results from several etiologies, which may be functional or anatomic. However, the main cause of BOO remains benign prostatic hyperplasia (BPH), secondary to BOO and carcinoma prostate. The complication of BOO can be devastating and long term. The resulting obstruction frequently produces lower urinary tract symptoms (LUTS) and becomes the main cause of lower urinary tract infections (LUTI). Material and Methods: In this cross-sectional study, a total of 100 consecutive patients of BOO who presented to our tertiary care hospital were enrolled in this study. After recording the demographic profile their mid-stream urine samples were collected and cultured for bacterial pathogens. The bacterial isolates were identified using standard microbiological methods and tested against a wide spectrum of antimicrobial agents using Kirby Bauer__ampersandsignrsquo;s method following the Clinical __ampersandsignamp; Laboratory Standards Institute (CLSI) guidelines. Results: Out of the 100 patients studied, 78% had BPH, and urine culture was positive in 74%. Most of these patients were more than 50 years of age group (90.5%) (Mean age= 61.2 years). There were 97.3% males and 2.7% of females. BPH was the most common cause of UTI as compared to the other causes of BOO (p=0.00001). Escherichia coli 46(62.1%) was the most common uropathogen causing UTI followed by Klebsiellapneumoniae 12(16.2%), Pseudomonas aeruginosa 10(13.5%), and the gram-positive organisms (Staphylococcus aureus, CoNS, Enterococcus faecalis 2.7% each). The study of their antimicrobial susceptibility showed that antimicrobial resistance to two or more drugs was present in the gram-negative (68/74) and grampositive (6/74) isolates. Conclusion: The present study shows that BPH continues to be the most frequent cause of BOO. There is a high prevalence of UTI in these patients which is caused by multidrug-resistance organisms. This study has important implications in the treatment of urinary tract infections among BOO patients in our region.