Introduction: A hospital-based study is conducted to find out the frequency, types, and aetiology and gender differences in brain lesions in patients diagnosed through Magnetic Resonance Spectroscopy (MRS) from South Kerala. Objectives: The study aims to assess the socio-demographic characteristics with the increasing incidence of brain lesions in patients attending a tertiary care hospital in South Kerala. Materials and Methods: In this study, there were 81 patients, both males and females, who underwent MRS for the diagnosis of brain lesions and studied for their socio-demographic characteristics which were recorded and compared. Results: The present study revealed that brain lesions were more common in males (57%) as compared to females (43%) (46:35). The frequency of brain lesions was higher in the middle aged group (52%) when compared to the younger age (6%) and old age group (42%). It is found that out of the 81cases, Meningioma is the most common types of brain lesions and also found that the symptoms are well correlating with clinical conditions as indicators for intervention. Conclusion: The analysis of the study indicates the need for special attention of such patients who come with early symptoms suggestive of brain lesions for the correct diagnosis through MRS and for further treatments.