Background: Pain is such an uncomfortable feeling that even a tiny amount of it is enough to ruin every enjoyment. Painful medical procedures such as immunizations and intravenous cannulation done in hospitals comprise a significant portion of the average child__ampersandsignrsquo;s experience with painful events. Inadequate relief from pain during childhood may have long term negative effects on future pain tolerance and pain response. Objective: To assess the effect of cartoon animation movie on the level of pain during intravenous cannulation among hospitalized children. Methodology: An experimental research design was used to assess the effect of cartoon animation movie on the level of pain during IV cannulation in 100 children of 2-7 years of age selected by convenience sampling. Results: The mean pain score during IV cannulation in the experimental group was lower (02.52 __ampersandsignplusmn; 03.37) than the control group (04.9 __ampersandsignplusmn;0 3.03) and this difference was statistically significant (p0.05). At three minutes after the IV cannulation procedure, the mean pain score in the experimental group was 0.00__ampersandsignplusmn;0.00 while in the control group was 02.43 __ampersandsignplusmn; 02.50 (p__ampersandsigngt;0.05). Conclusion: Cartoon animation movie significantly reduces the level of intravenous cannulation procedural pain in children in the experimental group as compared to the control group.