Introduction: Although the natural history of neck pain appears to be favourable, rates of recurrence and chronicity are high.As physiotherapists daily deal with all work-related musculoskeletal problems and the job nature of physiotherapists including treating the patients, lifting the body part of the patients and awkward posture to treat the patients leads to neck pain. Aqua stretch is a new form of assisted stretching in the water. It is considered a breakthrough in pain management and preventive medicine. Aqua stretch exercising seems to quickly dissolve/release fascial adhesions by controlling stretch resistance. Aim: To comparing the short term effect of aqua stretch with supervised land-based stretching among young working female physiotherapists having chronic non-specific neck pain. Methodology: 60 female physiotherapists with chronic neck pain age between 22-30 years of age were randomly allocated into 2 groups (aqua stretch and land-based stretching). After signing the consent form, patients were taken for pre-intervention NDI (neck disability index) score, NPRS (numerical pain rating scale) __ampersandsignamp; ROM (range of motion). Then instructions about aqua stretch were given. Treatment sessions will be 3 sessions per week and each session will be of 30 minutes up to 6 weeks. NDI questionnaire will be taken on the 1st meeting and again on weeks 4th and 6th. ROM will be measured weekly till 6 weeks, NPRS will be taken immediately after every session, twice a week, till 6 weeks. Result: Statistical significance (p__ampersandsignlt;0.05) was observed in the AquaStretch group for pain reduction (P= 0.006), ROM (P= 0.06), and NDI (P=0.0029). Conclusion: 6 weeks of Aquastretch can reduce pain, increase restricted ROM and also can improve quality of life in patients with chronic non-specific neck pain and also suggests that AquaStretch can improve pain tolerance in the cervical region.As a practical technique used to decrease pain and increase mobility AquaStretch could be a valuable addition to the current techniques available for chronic non-specific neck pain