Introduction: The seasonal variation of blood pressure has been demonstrated in various researches but the studies on the variation of blood pressure in students of the northern region of India is very sparse, also the data regarding the prevalence of prehypertension in Undergraduate students of the Varanasi region is lacking. Aims: This study aims to find the mean blood pressure variation of undergraduate students in the summer and winter season and to screen male and female students in the prehypertensive stage. Method: It was a longitudinal pilot study in which blood pressure of undergraduate students of Faculty of Ayurveda, Institute of Medical Sciences, Banaras Hindu University was measured in two different seasons i.e. summer and winter. A total of 50 students 25 (50%) males and 25 (50%) females of age 18 to 25 years were included in the study. Result and Conclusion: Mean of systolic blood pressure as well as diastolic blood pressure were significantly higher in the winter season as compared to the summer season. The mean difference between systolic blood pressure and diastolic blood pressure in the winter and summer season was 6.26 mmHg and 6.54mmHg, respectively. The mean of systolic blood pressure of males was significantly higher as compared to females in both winter and summer season by the difference of 11 mmHg in winter and 10 mmHg in summer which was statistically significant (P__ampersandsignlt;0.005)whereas the difference in diastolic blood pressure was found out to be non-significant. Out of 50 subjects, 19 (38%) subjects in the summer season and 25 (50%) subjects in the winter season were in the prehypertensive stage.