Aim: To examine the relationship of physical activity levels and physical fitness components with overweight and obesity in adolescent boys. Method: We recruited 332 school going boys of age 13-18 years as subjects. The subjects filled a validated questionnaire: Physical Activity Questionnaire-A (PAQ-A) to report their physical activity levels. They were further subjected to the testing of their physical fitness components including speed, explosive power, flexibility, agility, endurance, coordination, balance, handgrip strength and reaction time. The relationship between these variables and overweight and obesity were examined by applying the Point-biserial correlation test. Results: The prevalence of overweight and obesity was 5.42% and 1.51% respectively. Significant correlations were found between the Basal metabolic Index (BMI) status of adolescent boys and their physical activity levels and overall physical fitness status. Conclusion: Overweight and obese adolescent boys had inferior levels of physical activity and physical fitness profile than the non-overweight and obese boys.