Introduction: Cases of COVID 19 pandemic in India has been reported since 30th January 2020. Cases are detected by RTqPCR targeting one or more gene (E/S/N/RdRp/ORF1) based on ICMR guidelines. Results are to be reported as __ampersandsignlsquo;Inconclusive__ampersandsignrsquo; if only one target gene is detected in multiplex qPCR. Objective: The present study is aimed to analyze the __ampersandsignldquo;Inconclusive__ampersandsignrdquo; results based on the Ct value of the detected target gene, duration of symptoms of cases and by repeat testing with another fresh sample after 3 days. Methods: This cross-sectional observational study was conducted from June 2020 to December 2020 in a tertiary care hospital in Kolkata. Nasopharyngeal and throat swab from suspected cases were collected and sent to our laboratory for RT-qPCR. Results: A total of 94,443 samples were tested in our laboratory for seven months. 8,455 (8.95%) samples were found to be inconclusive. Out of them, 1287 (15.22%) cases became positive and 3858 (45.63%) became negative after retesting with another fresh sample after 3 days. 1540 (18.21%) cases were lost for follow up. All inconclusive results were correlated with Ct value and duration of the disease symptoms. Maximum cases (64.96%) with Ct value 36 became negative on retesting. Most of the cases (28.36%) had symptoms of less than 2 days duration, among positive cases, whereas, 36.86% cases had symptoms of more than 12 days duration among negative cases. 1770 (20.94%) samples were found to be inconclusive again on retesting after 3 days. Most of these cases (46.55%) had a Ct value within 34- 36 on the first test. Conclusion: All inconclusive samples should always be subjected to further testing after 3 days. There is a chance to get positive results from inconclusive cases whose Ct value is less than 34. Quality control and quality assurance of all processes should be done to check any pre-analytical or analytical fallacies. Clinicians and patients both are to be educated about the probable reasons of inconclusive also