Introduction: Diabetic lower limb wounds account for one of the commonest forms of complications with diabetes. These ulcerated lesions are easily susceptible to infections. Several studies have concluded the presence of varied microbial flora associated with the wounds. The mycology of the lesion is not given equal importance when compared to its bacterial aspect. Recent studies have shown the presence of wide range of fungal strains present in an infected foot ulcer of a diabetic patient, with Candida species accounting for the most commonly isolated strain. Aim: This study was done in an attempt to study the prevalence and spectrum of fungal infection in diabetic foot ulcers and to compare our findings with those of previous studies. Methodology: This hospital based prospective study. A total number of 100 patients with diabetic foot ulcers were included in this study. Patients already treated with anti-fungal therapy, chemotherapy, immunosuppressant, radiotherapy and corticosteroids were excluded. Detailed history of all patients was taken. These patients were evaluated with reference to clinical symptomatology and biochemical profile for diabetes mellitus. Two tissue samples, which were taken from the bed of the diabetic foot ulcer from each patient, one for microscopic examination and the other for culture sensitivity both bacterial and fungal. The patients were treated with regular dressing and antifungal therapy in addition to appropriate antibiotics as per bacterial culture sensitivity. The results were compared with other studies in literature and discussed. Results: Out of the 100 patients, evaluated 18% had positive fungal cultures, among them most common fungal strain was C__ampersandsignnbsp;albicans and secondly was C tropicalis Conclusion: Management of diabetic foot ulcers must include antifungal antibiotics as per fungal culture sensitivity report in addition to routine bacterial culture sensitivity and other modalities.