Background: Cochlear implantation is one of the most commonly used surgically techniques used in today__ampersandsignrsquo;s world for adults and children having sensorineural hearing loss. During cochlear implantation, there are surgical key steps that are influenced by the anatomical variation of each individual. Objective: The aim was to study the anatomical characteristics of human RW and its importance for electrode insertion for the preservation of residual hearing in the process of cochlear implantation (CI) surgery. Methods: Five human cadaveric temporal bones were obtained. Microdissection was done through facial recess approach and after dissection of the bone in the area of round and oval window (OW), height and width of the RW were noted, the distance between OW-RW was measured. Results: The average height and width of the RW were 0.76 + 0.065mm and 0.81 + 0.29 mm, respectively. The distance between OW-RW was 2.52 + 0.53 mm Conclusions: Electrode insertion could be challenging in cases where the height and width of RW are __ampersandsignlt; 1mm. This will help to select slimmer and less traumatic electrodes for cochlear implantation to avoid injury to neurovascular structures and preserve residual hearing.