Introduction: Reactive oxygen species produced in our body may result in the pathogenesis of different degenerative diseases like atherosclerosis, carcinogenesis, neurodegenerative diseases, ageing etc. Antioxidant-rich foods can protect us by neutralizing these free radicals reducing. Objective: This study determined the total flavonoid and phenolic content of various fruit and vegetable samples collected from a different region of Bhubaneswar, India. Methods: The methanolic extract of twelve fruits and vegetables were evaluated for their total phenolic content, total flavonoid content and antioxidant activity by DPPH scavenging assay. Results: The results showed high flavonoid and phenolic contents present in all the fruits and vegetable samples tested. These samples also exerted good antioxidant activity in the DPPH scavenging assay. There was a clear positive correlation between total flavonoid and phenolic content and a negative correlation between total flavonoid and phenolic content with IC50 value of DPPH radical scavenging assay based on the results. Conclusion: This work might be helpful to local consumers and nutritionists of the covered locality in selecting proper fruits and vegetables in their daily diet.