Introduction: Conventional push-ups on the ground are recognized for fairly improving shoulder strength. A fitness structure has been developed for push up for improved muscle activation. Objective: The present study aimed to compare the activity of the shoulder and trunk muscles in two push-up positions: push-ups on the fitness structure vs standard push-ups on the ground. Methods: The study was a comparative study conducted among physically fit adult males in which 18 volunteers were recruited from physical training organization. Participants received instructions on proper push-up position and technique. Participants did 10 push-ups each and Electromyography (EMG ) measures were recorded on biceps, triceps, deltoid anterior and pectoralis major muscle. These participants were asked to initially complete push-ups on the fitness structure followed by push up on the ground. The recordings of push-ups under the two conditions were analysed and compared using an unpaired t-test. Results: The mean age of the study participants was 26.94 __ampersandsignplusmn; 1.259. The activity of shoulder and trunk muscles was better in the case of push-ups on the structure than on the ground. The difference in mean EMG readings of biceps muscle (11.133 __ampersandsignplusmn; 0.871 vs 7.346 __ampersandsignplusmn; 1.121) (p__ampersandsignlt;0.001), triceps muscle (4.992 __ampersandsignplusmn; 0.881 vs 3.228 __ampersandsignplusmn; 0.438) (p__ampersandsignlt;0.001), deltoid muscle (5.328 __ampersandsignplusmn; 1.373 vs 3.257 __ampersandsignplusmn; 1.103) (p__ampersandsignlt;0.001) and pectoralis muscle (5.631 __ampersandsignplusmn; 1.290 vs 3.2906 __ampersandsignplusmn; 0.94875) (p__ampersandsignlt;0.001) was statistically significant. Conclusion: These results indicate that the designed structure could be a promising tool to those who perform rigorous physical activity. Future studies must include randomized trials to further validate our study results.