Introduction: Hepatitis means injury to the liver with inflammation of the liver cell. Most common cause of hepatitis in world is hepatitis viruses. Particular, types B and C virus lead to chronic disease in hundreds of millions of people. Hepatitis C virus is mostly transmitted through infective blood and blood products. Aim and Objective: This study done in order to known the status of Hepatitis C virus in clinically asymptomatic blood donors in blood bank of tertiary care hospital. Result: A total of 11333 blood donors were screened in last three years. Out of which 3646 blood units were screened in year 2011, 7 blood units were HCV reactive. Whereas 4266 blood units were screened in 2012, 6 blood units were HCV seropositive and in year 2013 total 3421 blood units were screened, out which 16 blood units were HCV seropositive. The average prevalence of hepatitis C virus was 0.25%. Conclusion: Blood transfusion is major route for transmission of HCV virus. This virus is greatest concern because of increasing burden of this disease and also life threatening for the recipients. This study is done to aware the public about status of HCV viral disease and prevents it mode of spread.