Zebrafish as the new emerging model is used to detail the study of the zebrafish, Taxonomy, Developmental stages of zebrafish. The various experimental model is used in the detection of the various function in the body, while In Vivo and In Vitro study, both studies are done under appropriate environment and proper procedure. Our present study mainly developing and testing medicines and vaccines for humans and other animals, studying how animals and human bodies function, Assessing the safety of chemicals such as pesticides for their possible effect on human health or the environment. In Pharmacological screening, various disease and disorder can be studied from the major disease which shows the better result in pharmacological screening. Teratogenicity is the most useful study as the embryo develops outside the body and the study of the embryo is done through the microscope. The other major property of zebrafish is the regeneration of the heart where the cardiovascular disease in the regeneration of the heart is studied. In Type-2 Diabetes Mellitus, the different test is studied to examine the abnormalities produce in the blood of zebrafish and the various effect of the drug which control the blood glucose level in the blood of zebrafish.