Introduction: Anatomy is one of the important subjects taught to the MBBS first-year students. Cadaveric dissection plays an important role in the deeper understanding of three- dimensional relationship of different anatomical structures, appreciating anatomical variations which are key to practice health and medicine. First-year medical students when encountering human cadavers for the first time faces lots of emotional and mixed feelings. The exposure has both physical and psychological impact on students. Objective: This study aimed to explore the knowledge and attitude of medical students regarding cadaveric dissection and anatomy as a career option. Methods: The study was conducted at Mayo institute of medical sciences, Barabanki. A total of 300 students of MBBS batch 2016 - 2017 and 2017- 2018 participated in this study. It is a questionnaire-based study done to evaluate student__ampersandsignrsquo;s behaviour, experiences and emotions regarding cadaver dissection and also to access their views on anatomy as a career option. Results: The emotional and physical stress encountered initially was decreased gradually after 3 months of dissection. It was found that students were not much interested in pursuing Anatomy as a career option due to a lack of opportunities. Conclusion: This study concludes that anatomical dissection remains an essential part of medical education and it should not be replaced by any other modern learning methods. The emotional shock experienced by the students can be greatly reduced if they are properly counselled before the dissection class. Research and job opportunities should be encouraged in the subject to develop interest among the young medical graduates for pursuing anatomy as a career.