Background: Helicobacter pylori are a gram-negative, curved bacillus, found in the gastric mucosa and play an important role in the development of gastritis, gastric ulcer, and gastric malignancy. More than half the world__ampersandsignrsquo;s population is infected with this organism. Because of this significant pathogenic diagnosis, H. Pylori is very essential. Various invasive and non-invasive methods are available for the identification of H.pylori. Histopathological identification of H.pylori can be improved with the use of special stains Objective: This study aimed to evaluate the efficacy of special stains like acridine orange, cresyl violet, Giemsa, toluidine blue, and warthinstarry stain in the identification of H.pylori compared to the routine H __ampersandsignamp;E stain (Haematoxylin and eosin). Methods: A Case Series Analysis of fifty gastric biopsies (twenty-five cases are positive for helicobacter pylori by Haematoxylin and Eosin stain and twenty-five gastric biopsies are negative for H pylori by the same method taken as controls) were included from March 2019-August 2019. Histological sections of gastric biopsy slides were stained with the H__ampersandsignamp;E, and five special stains and Sensitivity, Specificity was calculated Results: Out of 50 cases studied, the sensitivity of five special stains was excellent in both positive and negative cases of H.pylori diagnosed by H__ampersandsignamp;E stain. Conclusion: The most reliable stains are Cresyl violet, Acridine orange, Modified Giemsa, and Toluidine blue in terms of posi tivity, cost-effectiveness, time-consumption and can be used as an adjunct to standard H__ampersandsignamp;E stain for definitive confirmation of H.pylori in gastric biopsies.