Background: Pseudomonas syringae is a phytopathogen that is known to cause diseases in monocots and dicots worldwide. The strains of this pathogen are known for their host-specific interactions on different plant species. Objective: The present study focuses on a comparative analysis of the genomic architecture of strains of Pseudomonas syrin gae belonging to pathovars actinidiae and syringae. Methods: Complete genome sequences of nine strains of P. syringae were used for comparative analysis using computational tools. Sequence similarity and conservation in gene order was observed in the strains considered in this study. The analysis includes parameters like calculation of genomic subsets, synteny plots, conservation in gene order and phylogenetic analysis. Results: The observations helped to understand the genetic differences between taxonomically related pathogenic strains that have varying host responses. By implementing various bioinformatics tools and techniques, the differences in the genomic structure were evident, based on overall genome statistics, gene orthologs, paralogs, as well as the degree of rearrangement in the gene order. It was observed that strains belonging to the same pathovar share a great amount of genomic similarity within themselves, as compared to strains of different pathovars. Conclusion: The differences in the pathogenic habits and host specificity of the strains belonging to different pathovars, is evident from the basic differences in their genetic structure. The pathogenicity related genes such as Hrp, also show significant variation among their orthologues in both the pathovars. The extent of co-relation between host specificity and genomic organi sation can be estimated from the results obtained in this study.