Background: It is understood that ET suctioning has several complications. Despite this, the method of suctioning the ET persists without adequate justification for the specific methods used. Suctioning is a high-risk procedure that can lead to hypoxemia, vomiting, cardiovascular dysfunction, inflammation, atelectasis, increased intracranial pressure, and can also cause tracheal mucosal lesions. Those can be complications can be prevented by implementing the protocols regarding Endo-tracheal tube suction. Objective: The aim of this research is to assess staff Nurses__ampersandsignrsquo; current experience of Endo-Tracheal suctioning before introducing the protocol as well as to determine whether or not the protocol is successful. Methods: One Group Pre Test __ampersandsignndash;Post Test Design,the study was conducted in ICU__ampersandsignrsquo;s of selected hospitals among staff nurses. Results: It has been found that the Endo-tracheal suctioning protocol has been observed effective in improving knowledge and practice of Staff Nurses working in ICU.