Background: Diagnosis of extrapulmonary tuberculosis is often challenging due to the lack of a gold standard test. Cartridge based nucleic acid amplification testing (CBNAAT) has been advocated for rapid diagnosis of extra-pulmonary tuberculosis and to identify rifampicin resistance. Objective: To evaluate the diagnostic yield of Cartridge Based Nucleic Acid Amplification Testing (CBNAAT) in different forms of extrapulmonary tuberculosis and to compare its efficacy between different forms of EPTB. Methods: This is hospital-based prospective observational study conducted in the outpatient and indoor Respiratory Medi cine department. All adult patients of EPTB were recruited for the study and CBNAAT was performed from appropriate clinical specimens in all of them. Results were analyzed in light of the diagnostic yield of CBNAAT with special emphasis on comparing CBNAAT results between different groups of EPTB. Results: Out of 502 cases of EPTB, CBNAAT was positive in 138 (27.5%) patients. The mean age of the study population was 36.49__ampersandsignplusmn;14.05 years with 61.1% males. Tubercular meningitis (p-0.023) and tubercular empyema (0.04) were more prevalent in the under 30 years age group compared to other forms of EPTB. Sensitivity of CBNAAT was good for paravertebral abscess, tubercular empyema, tubercular meningitis, cold abscess and tubercular lymphadenitis, but sensitivity was very low in tuber cular pleural effusion and ascites. CBNAAT was significantly more positive in samples containing pus (70 out of 81, 86.41%; p-__ampersandsignlt;0.0001). Rifampicin resistance was detected in 14 cases, of which four also showed isoniazid resistance on Line Probe Assay Conclusion: CBNAAT adds significantly to the diagnostic yield of EPTB in comparison to conventional methods, but its sensitiv ity varies in different forms of extrapulmonary tuberculosis. It has the additional advantage of identifying rifampicin resistance with high sensitivity and specificity.