Background: Response to orthopaedic treatment modalities is maximum during the peak of the adolescent growth spurt. Objective: To assess serum insulin-like growth factor-1 levels ( IGF-1) in female and male subjects concerning various stages of cervical vertebrae maturation (CVMI). Methods: The study sample consisted of 240 subjects, 120 females and 120 males, in the age range of 8-16 years, who re ported for orthodontic treatment. Radiographs were assessed for different CVMI stages. After evaluating the X-rays for CVMI stages, subjects were placed into different groups; Group 1-CVMI 1 stage, Group 2- CVMI 2 stage, Group 3- CVMI 3 stage, Group 4- CVMI 4 stage, Group 5- CVMI 5 stage and Group 6- CVMI 6 stage, both for male and female subjects separately. Results: Evaluation of results revealed that the average age of male subjects in CVMI stage 1 was 10.15 years, in CVMI-2 was 11.76 yrs, in CVMI-3 was 13.04 yrs, in CVMI-4 was 14.29, in CVMI-5 was 15.45 and in CVMI-6 was 16.89. The average age of female subjects in CVMI stage 1 was 8.83 years, in CVMI-2 was 9.66 yrs, in CVMI-3 was 11.25 yrs, in CVMI-4 was 12.38, in CVMI-5 was 13.89 and in CVMI-6 was 15.12. Gender was a significant factor associated with the age of attainment of each CVMI stages. Male subjects showed on average delayed attainment of the CVMI stages by about 14 months later as a comparison to females. Conclusion: This study reveals that Serum IGF-1 levels are at peak in CVMI Stage 3 in both males and females. Biomarker IGF-1 serve as an alternate method for growth assessment which is non invasive to x-ray exposure , simple and gives a quantita tive value to find pubertal growth peak.