Introduction: Anaemia is one of the most common nutritional deficiency disorders affecting pregnant women; During pregnancy, the need for iron for mother and fetus gradually increases and will reach its highest level at the end of pregnancy. There should be awareness of anaemia among antenatal women and association of knowledge, attitude and practice of nutrition during pregnancy and taking iron-rich food. Objective: To assess the knowledge, attitude and practice regarding iron-rich diet and iron supplement to prevent anaemia in pregnancy in women of the rural region. Methods: The present study comprised 100 Second Trimester Pregnant Women. Pregnant women in the second trimester at tending Gynecology and Obstetrics OPD of AVBRH, Sawangi Wardha. Data was gathered by using a structured and pretested questionnaire which asked questions on nutrition in pregnancy, anaemia and associated factors. It has three parts: Knowledge, Attitudes and Practices. Results: The knowledge level of subjects was significantly correlated with their Haemoglobin levels. There was a significant positive correlation between the Attitude scores of subjects and Haemoglobin levels.No significant positive correlation was found between Practice Scores of Subjects and Haemoglobin levels. Conclusion: Present study concludes that the studied pregnant women had good knowledge and positive attitudes but poor practice toward prevention and the prevalence of iron deficiency anaemia. To address the issue of anaemia, the health service sector needs to incorporate health promotion strategies that will positively impact the attitude and practice levels of the popula tion.