Background: Regular participation in physical activity is essential to attain health benefits. Previous studies have stressed to consider domains of physical activity while assessing the physical activity levels across the different sections of the populations. Objective: To assess the differences in physical activity levels between males and females of the Majha region of Indian Punjab. Methods: A cross-sectional survey was conducted including the four districts of the Majha region of Indian Punjab. A total of 1130 participants including 628 females and 502 males were interviewed using the WHO-recommended Global Physical activity questionnaire (GPAQ). Physical activity was assessed in three domains viz. work, transport and recreational. Mann-Whitney U test was applied to compare the non-parametric data of physical activity levels. Results: No significant differences were found in the work domain and transport domain in any of the age group. However, the age group of 53-64 years and the overall sample showed significant differences between both genders (p__ampersandsignlt;.05). Conclusion: Males of the age group 53-64 years had higher levels of total physical activity and recreational activity than fe males.