Introduction: Dental plaque has been considered as the main factor in the initiation and progression of gingival and periodontal diseases. Various mouthwashes can be advised to reduce plaque and gingivitis. Objectives: This clinic microbiological study was done to evaluate the Cardamom extract and chlorhexidine on plaque and gingivitis in patients using an orthodontic appliance. Methods: The study included 40 subjects, with mild to moderate gingivitis and divided equally into 2 groups with 20 subjects in each as; Group 1-chlorhexidine gluconate mouthwash and Group 2- Cardamom extract mouthwash. Both the groups were directed to use 10 ml of mouthwash with an equal dilution of water for 1 min twice a day for thirty minutes after brushing. Param eters were noted for plaque and gingival index at day 0, 14 th, and 21 st day. The N-benzoyl-l-arginine-p- nitroanilide (BAPNA) assay was used to evaluate the trypsin-like activity of __ampersandsignldquo;red__ampersandsignrdquo; complex microorganisms. Results: On assessment between chlorhexidine and Cardamom extract mouthwash, the percentage reduction of the Plaque Index between 0 and 21 st day were 42.7336 and 42.7697, respectively (P=0.984), the percentage reduction of Gingival Index between 0 and 21 st day were 51.757and 50.757, respectively (P=0.934) and percentage reduction of BAPNA values at 21 days was 41.0345 chlorhexidine and, 52.178 for cardamom. Conclusion: Both cardamom and chlorhexidine mouth wash was helpful in the reduction of plaque and gingivitis.