Introduction: The literature to date has failed to establish a consensus concerning the relationship between postoperative pain and the number of treatment appointments. Some authors, support single-visit treatments, while others indicate that the use of such procedures can produce more discomfort for the patient and would therefore be less desirable under most circumstances. Objectives: This study aims to compare the incidence of post-obturation pain at one, two and seven days after single-visit and multiple-visit non-surgical endodontic treatments. Methods: 198 subjects have participated in the present study from the total sample size of 346. The sample was divided into two groups i.e Group-I with 93 subjects underwent the single sitting endodontic procedure and Group-II with 105 subjects underwent two sitting endodontic procedure, who reported back with the VAS reporting form. Pain score was recorded by using a Heft Parker visual analogue scale (VAS) method. Each patient was assigned a value between 0 and 170 on the VAS scale. Statistical analysis was done using SPSS 20 for Windows (SPSS Inc., Chicago, IL, USA) using Chisquare test at p__ampersandsignlt; 0.005. Results: There was no change in postoperative pain among subjects treated in one appointment and those treated in two ap pointments. Most of the patients in both groups conveyed no pain or only slight pain within 24 to 48 hours of treatment. Conclusion: Post-obturation pain after non-surgical endodontic therapy was not uncommon after one day, the second day but there was less pain or no pain after seven days. Among the teeth with post-obturation pain, the single-visit group had lower intensity pain, after one day, two days and after seven days, then the multiple-visit group had.