Introduction: A diagnostic technique that visualizes the upper portion of the gastrointestinal tract up to the duodenum is the upper gastrointestinal endoscopy. It is a proven model of investigation and plays a crucial role in the precise detection and treatment of a wide variety of upper gastro-intestinal conditions in conjunction with biopsy. Objectives: To study the Morphological pattern of lesions of the upper gastrointestinal tract from the endoscopic biopsies. Methods: The present research was performed with endoscopic biopsies of the upper gastrointestinal tract over two years in the pathology department of a tertiary care hospital. Results: In 828 patients, biopsy materials were collected from various levels of the upper gastrointestinal tract. This included 288 biopsies from the oesophagus (35%), 84 from the gastric junction of the oesophagus (10%), 444 from the stomach (54%) and 12 from the small intestine (2%). There were 280 (34%) cases that were non-neoplastic, while 548 (66%) were neoplastic. Histopathology revealed gastritis of varying grades (27%) as the inflammatory lesion most frequently diagnosed, while the most frequently diagnosed malignant lesion was squamous cell carcinoma (37 %). Conclusion: A strong correlation between endoscopic and histological diagnosis was shown in this research. It further indicates that the predominant site of upper gastro-intestinal malignancy is the oesophagus.