Introduction: Antibacterial effect of zinc oxide nanoparticles (ZnO-NPs) has remarked a huge interest overall especially by the usage of nanotechnology to synthesis particles in the nanometer size. ROS has been a central point for a few systems including cell wall destruction because of ZnO confined communication, improved film porousness, the disguise of NPs because of loss of proton intention power and take-up of poisonous broke up zinc particles. Objective: The antibacterial impact of zinc oxide (ZnO) nanoparticle and bacteriocin on Shigella species. Methods: Preliminary tests have been done for the identification of Lactobacillus acidophilus strains producing bacteriocin. Chloroform__ampersandsignndash;Methanol (2:1 v/v) was used for crude Bacteriocin extraction. However produced precipitate at Solvent-Aqueous interphase was collected aseptically, the solvent was evaporated and the precipitate was kept in a buffer which was used for the antimicrobial study. Sep-pack C18 cartridge and reverse-phase High-Performance Liquid Chromatography on a Nucleosil column were used for purification of lactation F. In this study Agar well diffusion and Broth dilution techniques were used to obtain antimicrobial activity of Lactation F. Synergistic antibacterial activity was done with 1: 1 combination of lactation f and ZnO nanoparticle against. Results: Lactacin F was purified and the antimicrobial testing was done with and without zinc oxide nanoparticle, which shows that Lactation F with zinc oxide nanoparticle had expanded zone of restraint to Shigella species when contrasted with Lactacin F alone. Conclusion: Invitro antibacterial activity of the synergistic compound was more effective than the crude extract of bacteriocin against Shigella species.