Background: Learning or computer-assisted teaching-learning is becoming popular at every stage of life. An animation is one of the computer applications and can have its impact on the teaching-learning process. Aims: The present study was designed to know whether the use of animation technique, in teaching can improve the process of understanding Methods: For this a group of 50 students was selected. The objectives of the study were explained to them. Pre-test was conducted by giving questionnaire. The topic was taught to them using overhead projector slides. Post test 1 was conducted giving same questionnaire. Then same topic was taught to them using animation tool. Post test 2 was conducted. The data obtained in the form of correct answers for all the three tests was analyzed statistically. At the same time feedback forms were given to all the students for purpose of analyzing the results qualitatively. Results: The results of pretest (P= NS) and posttest1 (p__ampersandsignlt;0.05) were compared (t= 4.8), and post-test1 p__ampersandsignlt;0.05) and post-test 2 (p__ampersandsignlt;0.001) were compared (t= 7.43) Discussion: After statistical analysis significant improvement in the knowledge was observed with the use of overhead projector slides, while highly significant improvement in knowledge and understanding was observed using animation tool. As per the results of feedback forms, 100% students agree that the animation is powerful tool to understand biological processes as it improves understanding and make learning interesting. Conclusion: Animation is a powerful tool than other traditional teaching tools, in understanding bioprocesses occurring in the cell.