The Kala is the structure that intervenes between the Dhatus(the vital tissues) and their Ashayas(the structure that holds the particular tissue). Kala is seven in number. As per the commentary by Acharya Dalhana, the PurishdharaKala is the AsthidharaKalawhich is described in visha-vegantara in kalpasthana. This relationship has also clinical importance in Ayurvedic treatment. This relation has not yet been studied. As Kala is the structure intervening Dhatu and it__ampersandsignrsquo;s Ashaya, the AsthiDhatu(bones) is directly related to AsthidharaKala, whereas the Purishdhara Kala is situated in the large intestine. A Research Protocol for Validation of __ampersandsignlsquo;Purishdhara Kala Sa Eva Ashtidhara Kala__ampersandsignrsquo; Using Unripe Bael Fruit (Aegle Marmelos Linn.) In Osteoporosis. In the present study, unripe Bael fruit (Aegle Marmelos Linn.) has been taken into account as an intervention because the Bael fruit is considered as the best remedy for gastrointestinal disorders, particularly the diseases of the large intestine. During this preclinical study, osteoporosis (OP) will be induced in Sprague Dawley rats with steroid. Unripe Bael fruit powder mixed with distilled water will be administered to the rats orally and transrectal. At the end of the study, the changes will be assessed on the following parameters. The histological changes in the colon and periosteum will be studied with the help of expert histopathologists. After resection of the portion of the colon, the mucosal surface will be graded with the help of a magnifying lens. A piece of the colon will be fixed in phosphate-buffered formaldehyde, embedded in paraffin, sectioned (4 __ampersandsignmu;m thick), and stained with hematoxylin and eosin. Each sample will be observed and evaluated under a light microscope by two independent observers. We anticipate that there will be an improvement in markers for osteoporosis after administration of unripe Bael fruit. Hence here is an effort to determine the protocol for the assessment of the relationship between Purishadhara and Asthidhara Kala.